MOPS
MOPS (3-(N-morpholino)propanesulfonic acid) is a buffer introduced by Good et al. in the 1960s. It is a structural analog to MES.[1] Its chemical structure contains a morpholine ring. HEPES is a similar pH buffering compound that contains a piperazine ring. With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.
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| Names | |
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| IUPAC name
3-Morpholinopropane-1-sulfonic acid | |
| Other names
3-(N-Morpholino)propanesulfonic acid, 3-Morpholinopropanesulfonic acid, 3-N-Morpholino propansulfonic acid, 4-Morpholinepropanesulfonic acid | |
| Identifiers | |
CAS Number |
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3D model (JSmol) |
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| ChemSpider | |
| ECHA InfoCard | 100.013.162 |
PubChem CID |
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| UNII | |
CompTox Dashboard (EPA) |
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InChI
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SMILES
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| Properties | |
Chemical formula |
C7H15NO4S |
| Molar mass | 209.26 g·mol−1 |
| Hazards | |
| Safety data sheet | MSDS |
| GHS pictograms |  |
| GHS Signal word | Warning |
GHS hazard statements |
H315, H319, H335 |
GHS precautionary statements |
P261, P264, P271, P280, P302+352, P304+340, P305+351+338, P312, P321, P332+313, P337+313, P362, P403+233, P405, P501 |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). | |
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| Infobox references | |
Applications
MOPS is frequently used as a buffering agent in biology and biochemistry. It has been tested and recommended for polyacrylamide gel electrophoresis.[2] Usage above 20 mM in mammalian cell culture work is not recommended.[3] MOPS buffer solutions become discolored (yellow) over time, but reportedly slight discoloration does not significantly affect the buffering characteristics.[4]
References
- Good, Norman E.; Winget, G. Douglas; Winter, Wilhelmina; Connolly, Thomas N.; Izawa, Seikichi; Singh, Raizada M. M. (1966). "Hydrogen Ion Buffers for Biological Research". Biochemistry. 5 (2): 467–77. doi:10.1021/bi00866a011. PMID 5942950.
- Thomas, J; Hodes, ME (1981). "A new discontinuous buffer system for the electrophoresis of cationic proteins at near-neutral pH". Analytical Biochemistry. 118 (1): 194–6. doi:10.1016/0003-2697(81)90178-0. PMID 6278979.
- Eagle, H. (1971). "Buffer Combinations for Mammalian Cell Culture". Science. 174 (4008): 500–3. doi:10.1126/science.174.4008.500. PMID 5110427.
- https://bostonbioproducts.com/products/mops-buffer-1-m-ph-74-bbm-74. Missing or empty
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External links
- Sigma Aldrich Buffer Calculator - Useful tool to calculate weight, volume, or concentration from molecular weight.
- Recipe for MOPS buffer on OpenWetWare